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CGP EDU Academic Team
Published on: August 14, 2026
is a test-tube reaction and does not involve the use of living cells. Its reaction is based on the temperature change, 
, at this temperature
Text Solution
Verified by ExpertsThe correct answer is:
B
Denaturation in PCR is done at 
for 
seconds.
At this temperature the hydrogen bonds that hold together the two polynucleotides of the double helix are broken, so the target DNA becomes denatured into single-stranded molecules.
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