E. coli is grown in a medium containing 
as the only nitrogen source for many generations. Then it is tranferred into a medium with normal 
. What could be the possible ratio of light and hybrid DNA after 
?
Text Solution
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According to semiconservative principle of DNA replication, after the completion of replication, each of DNA molecule would have one parental and one newly synthesised strand.
E. coli replicates every 20 minutes, when heavy DNA was given, it means it has two strands made up of 
(heavy isotope of nitrogen). There will be always 2 hybrid DNA strands at every generation, which were synthesised from the two strands of the heavy DNA. Therefore, after 60 minutes 6 light and 2 hybrid DNA will be obtained, hence there ratio will be 
.
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