A biology student is tasked with identifying the types of organic compounds present in living organisms. As part of a lab activity, she follows a classic biochemical protocol. She grinds a piece of liver in trichloroacetic acid using a mortar and pestle, forming a thick slurry. She then strains it using cotton and collects two distinct fractions:
Fraction A: The liquid that passes through the cotton
Fraction B: The material retained on the cotton
Based on the information provided, answer the following:
(I) Fraction A represents the acid-soluble pool.
(II) Fraction B represents the acid-insoluble fraction.
(III) Fraction A includes filtrate and contains larger macromolecules like proteins, nucleic acids, and polysaccharides.
(IV) Fraction B primarily includes retentate and contains small biomolecules such as amino acids, sugars, and vitamins.
Choose the correct option.
Text Solution
Verified by ExpertsB
• Fraction A represents the acid-soluble pool and it contains small biomolecules such as amino acids, sugars, and vitamins.
• Fraction B represents the acid-insoluble fraction and it contains large molecules such as proteins, nucleic acids, and polysaccharides.
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