RNA interference (RNAi) is a technique used in functional g…

Chemistry NEET Full Syllabus Mock Test 180 Question New Syllabus Mock Test - 8 PCB Single Correct MCQ

Published on: August 13, 2026

RNA interference (RNAi) is a technique used in functional genomics. Which of the following correctly explains its principle?

It uses antisense RNA molecules to promote transcription of a target gene.

It involves small RNA molecules that bind to complementary mRNA and prevent its translation.

It enhances gene expression by stabilizing the mRNA.

It blocks replication by binding to DNA polymerase.

Text Solution

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The correct answer is:

• RNA silencing involves small RNA molecules that bind to complementary mRNA and prevent its translation.

• RNAi works by silencing genes, i.e., reducing or blocking expression.

• Small RNAs (siRNA or miRNA) form complexes (RISC) that degrade or block translation of target mRNA.

• Antisense RNA does not promote transcription:

• Antisense RNA is complementary to the mRNA of a gene.

• When it binds to mRNA, it blocks translation (protein synthesis), not transcription.

• Transcription is the process of making RNA from DNA, and antisense RNA does not enhance this.

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