If alien DNA is introduced using SalI in pBR322 then transformant will grow on
Text Solution
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In pBR322, the Sall restriction site is located within the tetracycline resistance gene ( tet 
).
When foreign DNA is inserted at the Sall site:
The tet 
gene is inactivated → cells become sensitive to tetracycline.
The ampicillin resistance gene ( amp 
) remains functional → cells remain resistant to ampicillin.
Therefore, the transformants will grow on ampicillin-containing medium but not on tetracycline-containing medium.
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