Published by:
CGP EDU Academic Team
Published on: August 12, 2026
The below diagram refer to PCR. Identify the steps
A
, –Denaturation at 94-96°C, –Annealing at 94-96°C, –Extension through Taq polymerase at 40-60°C, –Amplification,
B
, –Annealing at 40-60°C, –Denaturation at 40-60°C, –Amplification, –Extension through Taq polymerase at 40-60°C,
C
and –Extension through Taq polymerase at 72°C, –Extension through Taq polymerase at 72°C, –Denaturation at 40-60°C, –Denaturation at 40-60°C,
D
. Select the correct option. 
–Amplified –Amplification –Annealing at 94-96°C – nnealing at 94-96°C
–Amplified –Amplification –Annealing at 94-96°C – nnealing at 94-96°C
Text Solution
Verified by ExpertsThe correct answer is:
A
Step : The DNA is being heated to separate the strands → Denaturation at 94–96°C
Step : Primers are binding to the single-stranded DNA → Annealing at 40–60°C
Step : DNA polymerase is synthesizing the new strand → Extension through Taq polymerase at 72°C
Step : Repeated cycles produce many copies → Amplified DNA.
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