Select the correct option which shows the most appropriate temperatures of three different steps of PCR Mechanism
Denaturation | Primer annealing | Primer extension | |
(a) | |||
(b) | |||
(c) | |||
(d) |
Text Solution
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Denaturation: This step needs a high heat of 94–96°C. This high heat splits the DNA strands apart.
Primer annealing: This step needs a lower heat of 40–60°C. This cool temperature lets primers stick to the DNA.
Primer extension: This step needs a medium heat of 72°C. This temperature helps the enzyme copy the DNA quickly.
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