Following are the steps of southern blot procedure.
(1) Autoradiography
(2) Hybridization with radioactive nucleic acid (probe)
(3) Blotting
(4) DNA fragments are treated to make them single stranded
(5) Electrophoresis
(6) Clearing of DNA by restriction endonuclease
(7) isolation of DNA from sample
The correct sequence is –
Text Solution
Verified by ExpertsB
Isolation of DNA from sample → (7)
Clearing DNA by restriction endonuclease → (6)
Electrophoresis of DNA fragments → (5)
DNA fragments treated to make them single-stranded → (4)
Blotting onto membrane → (3)
Hybridization with radioactive probe → (2)
Autoradiography to visualize bands → (1).
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