Home Biology Biotechnology Principles and Processes All Topic Question For Revision Test Which of the following is not correct about …
Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ
Published on: August 12, 2026

Which of the following is not correct about pBR 322 vector?

A
It was constructed by using DNA derived from naturally occurring plasmids of E coli.
B
It has two drug resistance genes-tet R and amp R .
C
It was developed by Bolivar and Rodriguez.
D
Selectable markers present in it can differentiate recombinants from non-recombinants on the basis of their inability to produce colour in the presence of chromogenic substrate.

Share this question

WhatsApp

For Instagram sharing, use “Apps” on mobile or copy the link.

Text Solution

Verified by Experts
The correct answer is:
D

This statement is incorrect because pBR322 does not use chromogenic substrates or color-based screening (like blue-white screening). That method is associated with vectors like pUC18/pUC19 using the lacZ gene and X-gal/IPTG system.

Correct → pBR322 is derived from natural E. coli plasmids

Correct → it has ampR and tetR antibiotic resistance genes

Correct → developed by Bolivar and Rodriguez

Incorrect → color-based screening is not a feature of pBR322.

──────────────────────────────────────────────────────────────────────────────────────────

Previous Question Next Question

Prepare Smarter with CGP Edu

Get practice questions, solutions, and test series in one place.

Write a Review

Share your experience with this question and solution.

Commentary

Send your comment, doubt, correction, or feedback to admin.

Student Reviews

What students say about this solution

No reviews yet. Be the first to write a review.

Similar Questions

Explore conceptually related problems

Plasmid has been used as vector because
Which of the following is known as specific molecular scissors?
Restriction enzymes belong to a larger class of enzymes, which is called as
There is a restriction endonuclease called EcoRI. What does.co part in it stand for?
Restriction endonuclease – Hind II always cuts DNA molecules at a particular point by recognizing a…
The enzyme used for joining two DNA fragments is called
CG
CGP Question Assistant Question Bank + AI Help
Hi! Type your question or upload one screenshot. First I will search related questions from CGP Edu Question Bank. If none match, type YES and I will solve it with AI.
Upload only one screenshot at a time. Flow: Question Bank first → If not matched, type YES for AI solution.