Plasmid pBR322 has Pstl restriction enzyme site within gene…

Biology NEET Previous Year Question Paper NEET 2021 Previous Year Question Paper Single Correct MCQ

Published on: August 13, 2026

Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain

it will be able to produce a novel protein with dual ability.

it will not be able to confer ampicillin resistance to the host cell.

the transformed cells will have the ability to resist ampicilin as well as produce

-galactoside.

it will lead to lysis of host cell.

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Since the gene is inserted at Pstl of ampR region ofpBR322, the ampR gene is inactivated which is called as insertional inactivation, Hence the genetically modified E.coli strain will not be able to confer ampicillin resistance.

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