Published by:
CGP EDU Academic Team
Published on: August 12, 2026
The correct sequence of making a cell competent is
A
treatment with divalent cations 
incubation of cells with recombinant DNA on ice 
heat shock 
placing on ice
incubation of cells with recombinant DNA on ice heat shock placing on ice
B
heat shock 
incubation of cells with recombinant DNA on ice 
treatment with divalent cations 
placing on ice
incubation of cells with recombinant DNA on ice treatment with divalent cations placing on ice
C
treatment with divalent cations 
placing on ice 
incubation of cells with recombinant DNA on ice 
heat shock 
placing on ice incubation of cells with recombinant DNA on ice heat shock
D
incubation of cells with recombinant DNA on ice 
heat shock 
treatment with divalent cations 
placing on ice.
heat shock treatment with divalent cations placing on ice.
Text Solution
Verified by ExpertsThe correct answer is:
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