Safranin is used as a counterstain in gram staining and endospore staining. It can also used for detection of cartilage, mucin and mast cell granule.
Carmine The basic dye, is used to stain nucleic acid and chromosomes, which possess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.
Basic Fuchsin: It involves staining of human chromosomes, elastic fibres, cardiac or skeletal muscle tissue.
Methylene blue: It used to stain nuclei, Golgi bodies and pectic substances.

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