Biology Biotechnology Principles and Processes Principles of Biotechnology and Restriction Enzymes Single Correct MCQ
Published on: August 14, 2026

First artificial DNA molecule was constructed by Cohen and (1) in (2) They used (3) enzyme to isolate the antibiotic resistance gene. They synthesized recombinant DNA by linking the antibiotic resistance gene with (4) DNA of (5) bacteria. Recombinant DNA with newly introduced antibiotic resistance gene was then transferred into another bacteria (6) .

A
(1)-Tatum, (3)-Restriction, (5)-E.coli
B
(4)-Plasmid, (6)-Salmonella
C
(2)-1972, (3)-Restriction, (6)-E.coli
D
(3)-DNA ligase, (4)-Plasmid, (5)-E.coli

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The correct answer is:
C

First recombinant DNA work was done in 1972.

Restriction enzymes were used to cut DNA.

Recombinant DNA was introduced into E. coli.

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