First artificial DNA molecule was constructed by Cohen and (1) in (2) They used (3) enzyme to isolate the antibiotic resistance gene. They synthesized recombinant DNA by linking the antibiotic resistance gene with (4) DNA of (5) bacteria. Recombinant DNA with newly introduced antibiotic resistance gene was then transferred into another bacteria (6) .
Text Solution
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First recombinant DNA work was done in 1972.
Restriction enzymes were used to cut DNA.
Recombinant DNA was introduced into E. coli.
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