Published by:
CGP EDU Academic Team
Published on: August 11, 2026
Which of the following enzyme will get inactivated in insertional inactivation?
A
Transacetylase
B
Permease
C
-galactosidase
D
Taq-polymerase
Text Solution
Verified by ExpertsThe correct answer is:
C
In insertional inactivation, foreign DNA is inserted into the lacZ gene, which encodes β-galactosidase. This insertion inactivates the enzyme.
• Insert present → β-galactosidase inactive → White colonies
• No insert → β-galactosidase active → Blue colonies
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