Published by:
CGP EDU Academic Team
Published on: August 14, 2026
Which of the following process will make the bacterial cells more competent so that they can take up plasmid DNA?
A
Bacterial cells are treated with specific concentrations of 
B
Bacteria are placed in hot water 
C
Bacteria are kept in moist environment
D
Bacteria are kept in dry environment
Text Solution
Verified by ExpertsThe correct answer is:
A
Bacterial cells are made competent (able to take up plasmid DNA) by treating them with specific concentrations of divalent cations like CaCl 2 (Ca 2+ ions). These ions help neutralize the negative charges on DNA and the cell membrane, allowing DNA entry during heat shock.
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