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Biology Biotechnology Principles and Processes Skill Builder Single Correct MCQ
Published on: August 14, 2026

Here are the recognition sites of some restriction endonucleases

Which of the above enzymes will not create compatible sticky ends?

A
( A) and and
B
and and

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The correct answer is:
C

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. This stickiness of the ends facilitates the action of the enzyme DNA ligase. In the given question, option A and C will produce sticky ends while option B and D will produce blunt ends.

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