Home Biology Biotechnology and Its Application Statement Based Questions Arrange the steps involved in developing a t…
Biology Biotechnology and Its Application Statement Based Questions Single Correct MCQ
Published on: August 12, 2026

Arrange the steps involved in developing a transgenic plant resistant to nematodes using RNA interference:

A
Production of both sense and anti-sense RNA in the host cells.
, , , ,
B
Silencing of the specific mRNA of the nematode, leading to the disruption of its survival.
, (E) , , , (E),
C
Introduction of nematode-specific genes into the host plant using Agrobacterium vectors.
, , , (E) ,
D
Formation of double-stranded RNA (dsRNA) in the host cells.
(E) Protection of the transgenic plant from the nematode. Choose the correct sequence from the options given below: , , (E) ,

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The correct answer is:
A

The process uses Agrobacterium vectors to insert nematode-specific genes into the host plant. This leads to the production of sense and anti-sense RNA, which combine to form double-stranded RNA (dsRNA). This dsRNA triggers RNA interference, silencing the corresponding nematode mRNA and disrupting its essential functions. As a result, the nematode cannot survive in the transgenic host, providing effective protection for the plant against the parasite.

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