Home Biology Biotechnology and Its Application Statement Based Questions Arrange the following steps used in gene the…
Biology Biotechnology and Its Application Statement Based Questions Single Correct MCQ
Published on: August 13, 2026

Arrange the following steps used in gene therapy for treating a child with adenosine deaminase (ADA) deficiency:

A
Introduction of the functional ADA cDNA into lymphocytes using a retroviral vector
B
Periodic infusion of genetically engineered lymphocytes into the patient
C
Culturing of lymphocytes outside the body
D
Extraction of lymphocytes from the patient's blood Choose the correct sequence of steps from the options given below:

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The correct answer is:
C

The process of gene therapy begins with the extraction of lymphocytes from the patient's blood , followed by culturing these lymphocytes outside the body . Next, a functional ADA cDNA is introduced into these lymphocytes using a retroviral vector , and finally, these genetically engineered lymphocytes are periodically infused back into the patient .

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