Insulin consists of two short polypeptide chains: chain and chain B, that are linked together by disulphide bridges. The formation of these disulphide bridges is a crucial step in the maturation of insulin. If the research team is having trouble with the final step of assembly, it could be due to issues in forming these bridges. The C peptide is not present in mature insulin and its removal is a part of the maturation process, not assembly. Similarly, creating a pro-hormone is a step prior to assembly. Isolating insulin from the host organism is a post-production step and not part of the assembly process.

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