Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ
Published on: August 13, 2026

If we added alien DNA at site Sal 1 in pBR322 than-

A
Transformed colonies become sensitive for tetracycline
B
Transformed colonies remains sensitive for ampicillin
C
Transformed colonies remains resistant for both tetracycline and ampicillin
D
Proteins involved in the replication of the plasmid does not formed

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Text Solution

Verified by Experts
The correct answer is:
A

In pBR322, the Sal I restriction site is located within the tet R (tetracycline resistance) gene. Insertion of foreign DNA at this site causes insertional inactivation of the tet R gene.

Ampicillin resistance (amp R ) remains intact.

Tetracycline resistance (tet R ) is lost.

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