Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ
Published on: August 12, 2026

In recombinant DNA technology, a plasmid vector must be cleaved by -

A
Four separate enzymes
B
Modified DNA ligase
C
A heated alkaline solution
D
The same enzyme that cleaves the donor genes

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Verified by Experts
The correct answer is:
D

In recombinant DNA technology, both the donor DNA (gene of interest) and the plasmid vector are cut using the same restriction endonuclease. This produces complementary sticky ends (or compatible ends) that can easily pair and be joined by DNA ligase.

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