Published by:
CGP EDU Academic Team
Published on: August 12, 2026
The correct sequence of making a cell competent is -
A
Treatment with divalent cations 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
) 🡪 🡪 placing on ice
) 🡪 🡪 placing on ice
B
Heat shock (42 
) 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
) 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
C
Treatment with divalent cations 🡪 🡪 placing on ice 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
)
)
D
Incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
) 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
) 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
Text Solution
Verified by ExpertsThe correct answer is:
A
(1) Treat bacterial cells with divalent cations 
to make them competent.
(2) Incubate with recombinant DNA on ice.
(3) Give heat shock at 
to facilitate DNA entry.
(4) Place on ice to stabilize the cells.
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