Which of the following is correct for molecular diagnosis?
(i) At a time when the symptoms of the disease are not yet visible, bacteria or virus can be detected by amplification of their nucleic acid by PCR.
(ii) PCR is now routinely used to detect HIV in suspected AIDS patients.
(iii) PCR being used to detect mutations in genes of suspected cancer patients.
(iv) PCR is a powerful technique to identify many genetic disorders.
(v) A double stranded DNA or RNA, tagged with a radioactive molecule (probe) is used to detect mutated gene by hybridsation followed by autoradiography.
Text Solution
Verified by ExpertsC
Let's check each statement:
(i) Correct - PCR can amplify nucleic acids of bacteria/viruses and detect infection before symptoms appear.
(ii) Correct - PCR is routinely used to detect HIV in suspected AIDS patients.
(iii) Correct - PCR is used to detect mutations in genes of suspected cancer patients.
(iv) Correct - PCR is a powerful technique for identifying many genetic disorders.
(v) Incorrect - The probe used in molecular diagnosis is typically a single-stranded DNA or RNA probe, not a double-stranded DNA or RNA molecule.
Therefore, the correct statements are (i), (ii), (iii), and (iv) only.
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