Recombinant DNA bearing foreign gene within the ampicillin resistance gene is passed in E.coli. The transformed and non-transformed cells are spread on agar plates containing ampicillin. Then
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The presence of restriction sites within the markers, e.g., 
and 
in pBR322, permits an easy selection for the transformed cells. When a foreign DNA segment inserts within the gene 
, the 
become non-functional due to insertional inactivation. Hence, in a medium containing ampicillin, the transformed cells will die and non-transformed cells will grow.
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