Sticky ends are short single-stranded overhangs created when DNA is cut by certain restriction endonucleases. These overhangs can easily pair with complementary sticky ends of DNA cut by the same enzyme. The pairing occurs via A between complementary nitrogen bases. Later on, DNA ligase permanently seals them by forming B in the sugar-phosphate backbone. Identify
and
respectively.
(A) | (B) | |
(a) | phosphodiester bonds | hydrogen bonds |
(b) | disulfide bonds | ester bonds |
(c) | hydrogen bonds | phosphodiester bonds |
(d) | ester bonds | hydrogen bonds |
Text Solution
Verified by ExpertsC
Sticky ends are short single-stranded overhangs created when DNA is cut by certain restriction endonucleases. These overhangs can easily pair with complementary sticky ends of DNA cut by the same enzyme. The pairing occurs via hydrogen bonds between complementary nitrogen bases. Later on, DNA ligase permanently seals them by forming phosphodiester bonds in the sugar-phosphate backbone.
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