Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements.
(I) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal.
(II) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
(III) Smaller the size of DNA fragment larger is the distance it travels through it.
(IV) Pure DNA can be visualised directly by exposing UV-radiation.
Select the correct option from the following.
Text Solution
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(I) DNA is loaded near the cathode (−), not the anode.
(II) DNA moves through the gel matrix, and gel concentration does affect its movement.
(III) Smaller DNA fragments travel a greater distance.
(IV) DNA cannot be seen directly under UV; it must be stained (e.g., with ethidium bromide).
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