Which of the following statements (i-iv) are correct. (i) I…

Biology Biotechnology Principles and Processes Single Correct MCQ

Published by: CGP EDU Academic Team

Published on: August 21, 2026

Which of the following statements (i-iv) are correct.

(i) In elution, the separated bands of DNA are cut out from agarose gel and extracted from the gel piece.

(ii) E. coli cloning vector pBR 322 shows several restriction, Ori antibiotic resistance genes and rop.

(iii) The dounstream processing and quality control testing vary from product to product.

(iv) Competent bacterial cell cannot take up the plasmid.

All of the above

None of the above

(i), (ii), and (iii)

Only (iv)

Text Solution

Verified by Experts

The correct answer is:

(i) Correct → In elution, DNA bands are cut from agarose gel and extracted.

(ii) Correct → pBR322 has ori, antibiotic resistance genes (ampR, tetR), and rop gene, plus restriction sites.

(iii) Correct → Downstream processing and quality control vary from product to product.

(iv) Incorrect → Competent bacterial cells are specifically capable of taking up plasmid DNA.

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