Home Biology Biotechnology Principles and Processes During the process of gene amplification usi…
Biology Biotechnology Principles and Processes Single Correct MCQ
Published on: August 21, 2026

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

A
Annealing
B
Extension
C
Denaturation
D
Ligation

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Text Solution

Verified by Experts
The correct answer is:
C

In PCR, high temperature is required first for denaturation to separate the DNA strands. If this step fails, the entire amplification process is affected.

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