Home Biology Biotechnology Principles and Processes In a cell, the separation of DNA strands is …
Biology Biotechnology Principles and Processes Single Correct MCQ
Published on: August 21, 2026

In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :

A
Two sets of Primers
B
Taq DNA polymerase
C
Deoxynucleotides
D
High temperature

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The correct answer is:
D

In PCR (Polymerase Chain Reaction), the separation of DNA strands is achieved by high temperature (~94–96°C) during the denaturation step.

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