Which of the following steps not involve in the catalytic cycle of an enzyme action?

(i) First, the substrate binds to the active site of the enzyme, fitting into the active site.

(ii) The binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate.

(iii) Enzyme-substrate complex is long-lived and required energy for dissociation.

(iv) Enzyme breaks the chemical bonds of the substrate and the new enzyme product complex is formed.

(v) The enzyme releases the products of the reaction and the free enzyme is ready to bind to another molecule of the substrate.