Restriction endonuclease enzyme cut the DNA fragments. These fragments are separated by gel electrophoresis in which ( A) charged DNA fragments are forced to move towards the ( B) under an electrical field through agarose gel. ( C) the size of DNA fragment, more it will travel.
(A) | (B) | (C) | |
(a) | Positively | Cathode | Smaller |
(b) | Negatively | Anode | Larger |
(c) | Positively | Cathode | Smaller |
(d) | Negatively | Anode | Smaller |
Text Solution
Verified by ExpertsD
DNA is negatively charged due to its phosphate groups.
During electrophoresis, DNA moves towards the positive electrode (anode).
Smaller DNA fragments move faster and travel farther through the agarose gel.
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