Published by:
CGP EDU Academic Team
Published on: August 21, 2026
Which of the following sequence is correct?
A
Incubation of cell with rDNA on ice 
treatment of divalent cation 
Heat shock 
placing on ice
treatment of divalent cation Heat shock placing on ice
B
Treatment of divalent cation 
Incubation of cell with rDNA on ice 
heat shock 
placing on ice
Incubation of cell with rDNA on ice heat shock placing on ice
C
Treatment of divalent cation 
heat shock 
Incubation of cell with rDNA on ice 
placing on ice
heat shock Incubation of cell with rDNA on ice placing on ice
D
None of the above
Text Solution
Verified by ExpertsThe correct answer is:
B
In the CaCl 2 heat shock method of transformation, the correct sequence is:
Treatment with divalent cations (Ca 2+ ) → makes bacterial cells competent
Incubation with recombinant DNA on ice → allows DNA to attach to cell surface
Heat shock → sudden increase in temperature helps DNA enter the cell
Placing back on ice → stabilizes the cell membrane
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