Home Biology Biotechnology Principles and Processes Statement Based Questions Which of the following are methods used to i…
Biology Biotechnology Principles and Processes Statement Based Questions Single Correct MCQ
Published on: August 21, 2026

Which of the following are methods used to introduce recombinant DNA into host cells?

A
Calcium treatment
, , ,
B
Micro-injection , and
C
Biolistics
and , and (F) , (E), and (F)
D
PCR
(E) Agarose gel electrophoresis
(F) Spooling Options:

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The correct answer is:
B

Calcium treatment(enhances cell permeability), microinjection (directly inserts DNA), and biolistics (shoots DNA-coated particles into cells) are techniques used to modify cells in such a way that DNA can be introduced into cells. PCR, Agarose gel electrophoresis and spooling are not for introducing rDNA into cells. PCR is used to amplify DNA, agarose gel electrophoresis is used to isolate and separate DNA and spooling is used to separate out DNA.

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