Arrange the following steps in the correct order as they occur in the synthesis of human insulin using recombinant DNA technology:
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The synthesis of human insulin starts with treating plasmid and human DNA (consisting of gene of interest) with restriction enzymes to prepare them for ligation , followed by the ligation of the insulin gene into a plasmid of E.coli . This recombinant DNA is then introduced into a bacterial cell . The bacteria produce insulin in a fermentation tank , and finally, insulin chains A and B are extracted and combined via disulfide bonds to form functional human insulin (E).
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