Home Biology NEET Full Syllabus Mock Test 180 Questions New Syllabus Mock Test - 14 PCB The following diagram shows the restriction …
Biology NEET Full Syllabus Mock Test 180 Questions New Syllabus Mock Test - 14 PCB Single Correct MCQ
Published on: August 21, 2026

The following diagram shows the restriction sites in E. coli cloning vector pBR322.

Find the role of ' X ' and ' Y ' genes.

A
The gene ' X ' is responsible for resistance to tetracyline and ' Y ' for proteins involved in the replication of plasmid.
B
The gene ' X ' is responsible for controlling the copy number of linked DNA and ' Y ' for proteins involved in the replication of plasmid.
C
The gene ' X ' is for proteins involved in the replication of plasmid and ' Y ' for resistance to antibiotic ampicillin.
D
Gene ' X ' is responsible for origin of replication and ' Y ' is responsible for antibiotic resistance

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The correct answer is:
C

The gene " X " in the plasmid vector pBR322 indicates 'rop' that codes for the proteins involved in the replication of plasmid and Gene "Y" in the plasmid codes for resistance to ampicillin antibiotic.

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