Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ
Published on: August 21, 2026

If we insert foreign DNA in pBR322 by the help of EcoRI than we can identify recombinant cells with the help of -

A
Insertional inactivation of amp r site
B
Insertional inactivation of tet r site
C
Insertional inactivation of β-galactosidase site
D
None of these methods

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The correct answer is:
D

In pBR322, the EcoRI site is not located within the amp R or tet R genes. Therefore, insertion of foreign DNA at the EcoRI site does not cause insertional inactivation of either resistance gene, so recombinants cannot be identified by these methods.

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