Which of the following is not correct about pBR 322 vector? | CGP Edu

Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ

Published by: CGP EDU Academic Team

Published on: August 21, 2026

Which of the following is not correct about pBR 322 vector?

A

It was constructed by using DNA derived from naturally occurring plasmids of E.coli

B

It has two drug resistance genes-tet R and amp R

C

It was developed by Bolivar and Rodriguez

D

Selectable markers present in it can differentiate recombinants from non-recombinants as recombinants are able to produce colour in the presence of chromogenic substrate

Text Solution

Verified by Experts

The correct answer is:

Color-based screening (blue-white screening using lacZ) is a feature of pUC vectors, not pBR322. In pBR322, recombinants are identified by insertional inactivation of antibiotic resistance genes, not by color production.

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