Published by:
CGP EDU Academic Team
Published on: August 21, 2026
The correct sequence of making a cell competent is -
A
Treatment with divalent cations 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
) 🡪 🡪 placing on ice
) 🡪 🡪 placing on ice
B
Heat shock (42 
) 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
) 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
C
Treatment with divalent cations 🡪 🡪 placing on ice 🡪 🡪 incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
)
)
D
Incubation of cells with recombinant DNA on ice 🡪 🡪 heat shock (42 
) 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
) 🡪 🡪 treatment with divalent cations 🡪 🡪 placing on ice
Text Solution
Verified by ExpertsThe correct answer is:
A
(1) Treat bacterial cells with divalent cations 
to make them competent.
(2) Incubate with recombinant DNA on ice.
(3) Give heat shock at 
to facilitate DNA entry.
(4) Place on ice to stabilize the cells.
Prepare Smarter with CGP Edu
Get practice questions, solutions, and test series in one place.
Write a Review
Share your experience with this question and solution.
Commentary
Send your comment, doubt, correction, or feedback to admin.
Similar Questions
Explore conceptually related problems
Cry gene codes for –
If we added alien DNA at site Sal 1 in pBR322 than-
Chilled ethanol is used for –
Sticky ends named so because -
Ethidium bromide stained DNA in agarose gel appear bright orange when it is exposed to
For heat shock competent cells and recombinant DNA are briefly placed at ________ temperature.