Read the following and choose the option with all the correct statements.

(i) At present, about 30 recombinant therapeutics have been approved for human-use world over. In India, 12 of these are presently being marketed.

(ii) Insulin used for diabetes was earlier extracted from pancreas of slaughtered cattle and pigs.

(iii) Insulin consists of two long polypeptide chains chain A and chain B, that are linked together by disulphide bridges.

(iv) In humans, the pro-hormones of insulin contains an extra stretch called C peptide and so needs to be processed before it becomes a fully mature and functional hormone.

(v) In 1938, Eli lilly an American company prepared tow DNA sequences corresponding to chain A and Chain B of human insulin and introduced them in plasmid of bacterium E.coli to produce insulin chains.

(vi) The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency

(vii) In some children ADA deficiency can be cured permanently by bone marrow transplantation; in others it can be treated by enzyme replacement therapy.

(viii) In gene therapy for ADA deficiency, a functional ADA cDNA using a retroviral vector is introduced in erythrocytes.

(ix) In ADA deficiency, if the gene isolated from marrow cells producing ADA is introduced into cells at early embryonic stages, it could be a permanent curve.

(x) Some important techniques that serve the purpose of early diagnosis of disease are recombinant DNA technology, PCR and ELISA.