Genetic engineering or recombinant DNA technology requires key tools such as restriction enzymes, polymerase enzymes, ligases, vectors, and the host organism.
Given below are two statements:
Statement I: Restriction endonucleases remove nucleotides either from 
or 
ends of DNA molecules.
Statement II: Selection of transformants from non-transformants can be done by plating on agar rich media with tetracycline, if insertional inactivation takes place in 
I recognition site of cloning vector pBR322.
In the light of the above statements, choose the most appropriate answer from the options given below:
Text Solution
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(i) Restriction endonucleases cut DNA at specific recognition sequences within the DNA, not from the ends. Restriction endonucleases remove nucleotides from the centre of dsDNA.
(ii) Exonucleases remove nucleotides from the ends of dsDNA either from 3' or 5' ends of DNA.
(iii) If insertional inactivation take place in Pvu I recognition site of the cloning vector pBR322, then plasmid will loose its resistance to the ampicillin antibiotic. However, transformants containing recombinants can be selected from non-transformants as they will grow in tetracycline media but not in ampicillin.
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