The different steps involved in the process of recombinant DNA technology are given below randomly? Arrange these in correct order.
(I) Extraction of the desired gene product.
(II) Amplification of the gene of interest.
(III) Isolation of a desired DNA fragment.
(IV) Ligation of the DNA fragment into a vector.
(V) Insertion of recombinant DNA into the host.
Correct order is
Text Solution
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The correct sequence in recombinant DNA technology is:
(III) – Isolation of the desired DNA fragment
(II) – Amplification of the gene of interest (e.g., by PCR)
(IV) – Ligation of DNA fragment into a vector
(V) – Insertion of recombinant DNA into the host
(I) – Extraction of the desired gene product (protein or RNA).
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