Identify the correct statements.
(I) The first recombinant DNA was constructed by using a piece of DNA from plasmid carrying anti biotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of E. coli.
(II) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligases.
(III) The presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
Text Solution
Verified by ExpertsA
(I) First recombinant DNA was made using DNA from Salmonella typhimurium linked to E. coli plasmid – True. This is historically correct (Cohen & Boyer, 1973).
(II) DNA fragments cut by the same restriction enzyme have compatible sticky ends and can be joined by ligase – True. This is the basis of recombinant DNA construction.
(III) More than one recognition site in the vector generates several fragments, complicating cloning – True. Multiple cuts make it harder to insert a gene precisely.
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