Biology Biotechnology Principles and Processes Cloning Vectors and Competent Host Single Correct MCQ
Published on: August 11, 2026

Restriction endonucleases hydrolyzes polynucleotide from

A
only the 5' end
B
from either terminal
C
at an internal phosphodiester bond
D
a phosphodiester bond within a specific sequence

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Verified by Experts
The correct answer is:
D

Restriction endonucleases recognize specific DNA sequences (restriction sites) and cut the DNA by breaking the phosphodiester bond within those sequences.

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