Biology Biotechnology Principles and Processes Cloning Vectors and Competent Host Single Correct MCQ
Published on: August 11, 2026

Which of the following processes require energy?

A
ligation
B
transformation
C
restriction digestion
D
hybridization

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Text Solution

Verified by Experts
The correct answer is:
A

Ligation requires energy in the form of ATP (or NAD⁺ in some bacteria) for joining DNA fragments.Transformation is uptake of DNA by cells and does not directly require ATP for the joining process.

Restriction digestion is carried out by restriction enzymes and does not require ATP. Hybridization is the pairing of complementary nucleic acid strands and does not require ATP.

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