Which statement correctly describes sequential steps in cDN…

Biology Biotechnology Principles and Processes Cloning Vectors and Competent Host Single Correct MCQ

Published on: August 11, 2026

Which statement correctly describes sequential steps in cDNA cloning?

reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector

mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector

double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Text Solution

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The correct answer is:

mRNA is isolated from cells.

Reverse transcriptase makes the first cDNA strand.

Second DNA strand is synthesized.

cDNA ends are modified (linkers/restriction sites added).

cDNA is ligated into a vector.

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