Home Biology Biotechnology Principles and Processes NCERT Direct visual selection method for the selec…
Biology Biotechnology Principles and Processes NCERT Single Correct MCQ
Published on: August 13, 2026

Direct visual selection method for the selection of recombinant host cells on the basis of their inability to produce, colour in the presence of chromogenic substrate can be applied if the vector used is a

A
Modified plasmid vector of E. coli
B
Modified bactedophage
C
Modified Agrobacterium tumifaciens plasmid
D
Disarmed retroviruse

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Verified by Experts
The correct answer is:
A

The blue-white screening method uses the lacZ (β-galactosidase) reporter gene in modified E. coli plasmid vectors. Recombinant colonies cannot produce the blue color in the presence of a chromogenic substrate (X-gal), so they appear white.

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