Identify the properties of a good vector used in rDNA technology.
(i) It should have origin of replication supporting high copy number.
(ii) It should have preferably more than '2' recoginition sites.
(iii) the restriction sites in vector should be in the antibiotic resistant genes.
(iv) It should have suitable marker genes.
(v) It should be easy to isolate and purify.
Choose the most appropriate answer from the options given below:
Text Solution
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(i) True — Good vector should have an origin of replication that supports high copy number.
(ii) False — Vector should have single or few restriction sites, not more than 2.
(iii) True — Restriction sites are often present within antibiotic resistance genes for insertional inactivation (selection).
(iv) True — Marker genes are required for selection of transformants.
(v) True — Vector should be easy to isolate and purify.
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