Two enzymes responsible for restricting the growth of bacteriophage in E.coli were isolated in 1963, one of these cut DNA, while other :
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In E. coli, two important enzymes involved in restriction-modification system were discovered in 1963:
One enzyme cuts foreign (bacteriophage) DNA → restriction endonuclease
The other enzyme protects host DNA by adding methyl groups (–CH₃) to specific DNA sequences → methylase (methyltransferase)
Methylation prevents the host DNA from being cut by its own restriction enzymes.
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