Home Biology Biotechnology Principles and Processes NCERT If DNA is inserted within the coding sequenc…
Biology Biotechnology Principles and Processes NCERT Single Correct MCQ
Published on: August 11, 2026

If DNA is inserted within the coding sequence of β-galactosidase enzyme then

A
Non-recombinants will give blue coloured colonies in presence of chromogenic substrate
B
Recombinant will give blue coloured colonies in presence of chromogenic substrate
C
Both recombinants and non-recombinants give blue colour
D
Non-recombinants do not produce colour due to insertional inactivation.

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Verified by Experts
The correct answer is:
A

When DNA is inserted into the β-galactosidase (lacZ) gene, it causes insertional inactivation, so recombinant bacteria cannot produce functional enzyme and give white colonies, while non-recombinants produce blue colonies in the presence of chromogenic substrate (like X-gal).

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