Correct statements are:
( A) Bacteria are treated with a specific concentration of divalent cations, such as barium, for transformation.
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Text Solution
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The disarmed method allow to infect the cell, transfer the recombinant DNA into host cell.
The cell normally does not take the foreign gene, a bacterial cell is made competent enough to take up the DNA. The efficiency with which DNA penetrates the bacterial cell through holes in the cell wall is increased by treating the bacterial cell with a certain concentration of a divalent cation, such as calcium. Another technique for injecting DNA straight into an animal's nucleus is known as microinjection.
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